TB Diagnostics

The Tuberculosis (TB) laboratory was previously housed at the National Microbiology Reference Laboratory (NMRL) through a Memorandum of Understanding (MoU) with the Ministry of Health and Child Care (MoHCC) to carry out capacity building of TB diagnostics testing and training of the NMRL personnel and to eventually set up the country’s second National TB laboratory.  Through this MoU BRTI was responsible for the running of a Bio-Safety Category lll TB laboratory in collaboration with the MoHCC, where TB culture, identification and drug susceptibility testing (DST) were performed.  Apart from capacity building, the BRTI TB Lab unit also contributes towards the national TB Drug Resistance statistics.  The laboratory is now housed at number 10 Seagrave Road Avondale, Harare, Zimbabwe. Since the relocation to the new site the laboratory is currently running the following methods for TB diagnosis;

Microscopy

    -Fluorescent staining – Auramine

    -Ziehl Nielsen staining

• Culture

    -Solid LJ

    -Manually read MGIT

Drug Susceptibility Testing (DST)

    -1st and 2nd line TB DST on solid agar

•Microscopic Observation Drug Susceptibility (MODS) Assay 

GeneXpert – Ultra

Rapid methods for the detection of TB (LAM Fuji) and TB drug resistance using Next Generation Sequencing (NGS) are being evaluated.