A Centre of Excellence for Biomedical Research and Training In Africa

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TB Diagnostics

TB laboratory which was housed at the National Microbiology Reference Laboratory (NMRL) through an MOU with MoHCC to carry out capacity building of the NMRL personnel and eventually set up the country’s second National TB laboratory.  Through this MoU BRTI was responsible for the running of a Bio-Safety Category 3 TB laboratory in collaboration with the MoHCC, where TB culture, identification and drug susceptibility testing (DST) were performed.  Apart from capacity building, the BRTI TB Lab unit also contributes towards the national TB Drug Resistance statistics.  The laboratory is now housed at number 10 Sea grave Road Avondale. Since the relocation to the new site the laboratory is currently running Gene expert for TB diagnosis. Rapid methods for the detection of TB (LAM Fuji) and TB drug resistance (NGS) will be evaluated.


Microscopy

• Fluorescent staining – Auramine

• Ziehl Nielsen staining

• Culture

• Solid LJ

• Manually read MGIT

• Drug Susceptibility Testing (DST)

• 1ST and 2nd line DST on solid agar

• MODS

• GeneXpert

TB PicFig 1. CAT III TB Laboratory facility

TB PicFig 2. TB Blood and Sputum MGIT Cultures incubating in a 37oC Incubator